Characterisation of Transcripts from the Human Cytomegalovirus Genes TRL7, UL20a, UL36, UL65, UL94, US3 and US34
Identifieur interne : 003408 ( Main/Exploration ); précédent : 003407; suivant : 003409Characterisation of Transcripts from the Human Cytomegalovirus Genes TRL7, UL20a, UL36, UL65, UL94, US3 and US34
Auteurs : Gillian M. Scott [Australie] ; Barclay G. Barrell [Royaume-Uni] ; Jon Oram [Royaume-Uni] ; William D. Rawlinson [Australie]Source :
- Virus Genes [ 0920-8569 ] ; 2002-01-01.
English descriptors
- KwdEn :
- TRL7, UL20a, UL36, UL65, UL94, US3, US34, cytomegalovirus, transcription.
Abstract
Abstract: The genome of human cytomegalovirus (HCMV) has been studied extensively in some regions, but not others. In this study, transcripts of the genome were further characterised for open reading frames (ORFs) TRL7, UL36, UL65, UL94, US3 and US34, and for the previously unrecognised ORF, UL20a. Reverse transcription-PCR demonstrated the presence of spliced transcripts from the putative glycoprotein gene, UL20a, at early and late times post-infection. US3 full-length and spliced transcripts, including a previously unidentified transcript (US3ii), were described at immediate early times. Sequencing of the complete ORFs of UL20a and US3 from 21 clinical isolates showed that US3 is well conserved in all isolates (97–100% identity), whereas UL20a shows more variation at the nucleotide level, with 90–100% identity. The limits of transcription, and splice donor and acceptor sequences for UL20a and US3 were conserved in all isolates, indicating likely conservation of mRNA splicing patterns. Sequencing a late cDNA library identified the limits of transcription for ORFs TRL7, UL94 and US34 and transcription from the TRL7 ORF was confirmed by northern blotting. Transcripts were found that were congruent with UL36 and UL65, but these differed in the limits previously predicted for these ORFs. These findings show the variation between predicted and actual transcription and indicate the complex nature of transcription from HCMV ORFs.
Url:
DOI: 10.1023/A:1014033920070
Affiliations:
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Scott</name><affiliation wicri:level="1"><country xml:lang="fr">Australie</country><wicri:regionArea>Virology Division, Department of Microbiology, SEALS, Prince of Wales Hospital, 2031, Randwick, NSW</wicri:regionArea><wicri:noRegion>NSW</wicri:noRegion></affiliation></author><author><name sortKey="Barrell, Barclay G" sort="Barrell, Barclay G" uniqKey="Barrell B" first="Barclay G." last="Barrell">Barclay G. Barrell</name><affiliation wicri:level="1"><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>The Sanger Centre, Hinxton Hall, CB10ISA, Hinxton</wicri:regionArea><wicri:noRegion>Hinxton</wicri:noRegion></affiliation></author><author><name sortKey="Oram, Jon" sort="Oram, Jon" uniqKey="Oram J" first="Jon" last="Oram">Jon Oram</name><affiliation wicri:level="1"><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Division of Virology, PHLS Centre for Applied Microbiology and Research, Porton Down, SP40JG, Salisbury, Wiltshire</wicri:regionArea><wicri:noRegion>Wiltshire</wicri:noRegion></affiliation></author><author><name sortKey="Rawlinson, William D" sort="Rawlinson, William D" uniqKey="Rawlinson W" first="William D." last="Rawlinson">William D. Rawlinson</name><affiliation wicri:level="1"><country xml:lang="fr">Australie</country><wicri:regionArea>Virology Division, Department of Microbiology, SEALS, Prince of Wales Hospital, 2031, Randwick, NSW</wicri:regionArea><wicri:noRegion>NSW</wicri:noRegion></affiliation><affiliation wicri:level="1"><country wicri:rule="url">Australie</country></affiliation></author></analytic><monogr></monogr><series><title level="j">Virus Genes</title><title level="j" type="abbrev">Virus Genes</title><idno type="ISSN">0920-8569</idno><idno type="eISSN">1572-994X</idno><imprint><publisher>Kluwer Academic Publishers</publisher><pubPlace>Boston</pubPlace><date type="published" when="2002-01-01">2002-01-01</date><biblScope unit="volume">24</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="39">39</biblScope><biblScope unit="page" to="48">48</biblScope></imprint><idno type="ISSN">0920-8569</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0920-8569</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>TRL7</term><term>UL20a</term><term>UL36</term><term>UL65</term><term>UL94</term><term>US3</term><term>US34</term><term>cytomegalovirus</term><term>transcription</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: The genome of human cytomegalovirus (HCMV) has been studied extensively in some regions, but not others. In this study, transcripts of the genome were further characterised for open reading frames (ORFs) TRL7, UL36, UL65, UL94, US3 and US34, and for the previously unrecognised ORF, UL20a. Reverse transcription-PCR demonstrated the presence of spliced transcripts from the putative glycoprotein gene, UL20a, at early and late times post-infection. US3 full-length and spliced transcripts, including a previously unidentified transcript (US3ii), were described at immediate early times. Sequencing of the complete ORFs of UL20a and US3 from 21 clinical isolates showed that US3 is well conserved in all isolates (97–100% identity), whereas UL20a shows more variation at the nucleotide level, with 90–100% identity. The limits of transcription, and splice donor and acceptor sequences for UL20a and US3 were conserved in all isolates, indicating likely conservation of mRNA splicing patterns. Sequencing a late cDNA library identified the limits of transcription for ORFs TRL7, UL94 and US34 and transcription from the TRL7 ORF was confirmed by northern blotting. Transcripts were found that were congruent with UL36 and UL65, but these differed in the limits previously predicted for these ORFs. These findings show the variation between predicted and actual transcription and indicate the complex nature of transcription from HCMV ORFs.</div></front></TEI><affiliations><list><country><li>Australie</li><li>Royaume-Uni</li></country></list><tree><country name="Australie"><noRegion><name sortKey="Scott, Gillian M" sort="Scott, Gillian M" uniqKey="Scott G" first="Gillian M." last="Scott">Gillian M. Scott</name></noRegion><name sortKey="Rawlinson, William D" sort="Rawlinson, William D" uniqKey="Rawlinson W" first="William D." last="Rawlinson">William D. Rawlinson</name><name sortKey="Rawlinson, William D" sort="Rawlinson, William D" uniqKey="Rawlinson W" first="William D." last="Rawlinson">William D. Rawlinson</name></country><country name="Royaume-Uni"><noRegion><name sortKey="Barrell, Barclay G" sort="Barrell, Barclay G" uniqKey="Barrell B" first="Barclay G." last="Barrell">Barclay G. Barrell</name></noRegion><name sortKey="Oram, Jon" sort="Oram, Jon" uniqKey="Oram J" first="Jon" last="Oram">Jon Oram</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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